Figure 1. PHLDA2 expression in CRC tissue, adjacent normal tissue, and cell lines. (A–C) Immuno-histochemical staining and analysis of PHLDA2 protein in CRC tissue and adjacent normal tissue (magnification, ×100 and ×400). (D) RT-qPCR was used to detect mRNA expression levels of PHLDA2 in 29 CRC tissues and paired normal tissues. (E–G) RT-qPCR and western blot analyses were used to detect mRNA and protein expression of PHLDA2 in six CRC cell lines. Data are shown as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001.