Figure 3. Low PHLDA2 expression inhibits CRC cellular migration and invasion by down regulation of EMT. (A, B) Invasion and migration of HCT116 and SW480 cells were measured by flow cytometry. (C) Western blots showing the levels of EMT-associated proteins; Vimentin, N-cadherin, E-cadherin, β-catenin, and MMP2. (D, E) Protein expression and sub-localization of E-cadherin as visualized by immunofluorescence. Data are shown as mean ± SD; *P < 0.05, ** P< 0.01, and ***P < 0.001.