Research Paper Volume 12, Issue 9 pp 7985—8000

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway

Figure 4. Inhibition of PHLDA2 induces cell cycle arrest in G1/S phase and increases apoptosis in CRC cells. (A, B) Apoptosis and cell cycle phase of transfected cells as measured by flow cytometry. (C) Western blots showing the levels of apoptosis-associated proteins (Cleaved caspase-3, Cleaved PARP, Bax, and Bcl-2) were assessed. Data are shown as mean ± SD; *P < 0.05, ** P< 0.01, and ***P < 0.001.