Figure 5. Low PHLDA2 expression enhances autophagic flux in CRC cells. (A, B) We used TEM (×20,000) to image HCT116 and SW480 cells and quantified the number of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. Red arrows, autophagic vacuoles; *P<0.05, ***P<0.001. (C, D) Expression of autophagy-associated proteins ATG7, Beclin1, LC3, and P62. Data are shown as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001.