Research Paper Volume 12, Issue 9 pp 7985—8000

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway

Figure 6. PHLDA2-mediated autophagy contributes to apoptosis induced by PHLDA2 inhibition in of CRC cells. (A, B) We used flow cytometry to detect cellular apoptosis with or without PHLDA2 inhibition, as well as with or without autophagy suppression by chloroquine (CQ). (CE) Protein levels of LC3 and p62 were assessed by western blot with or without PHLDA2 inhibition, as well as with or without autophagy suppression by CQ. Data are shown as mean ± SD; ns: no significant difference; *P < 0.05, **P < 0.01, and ***P < 0.001.