Figure 1. The proliferation of NSPCs was promoted by the ART. (A, B) CCK8 was used to examine the effects of different ART dosages (0, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8 and 25.6 μmol/L) on the proliferation of neural stem cells at 24h and 72h post-treatment. (C, D) Western blotting was used to detect the expression level of Nestin in NSPCs treated with ART (0, 0.4, 0.8 and 1.6 μmol/L). (E) RT-qPCR was used to examine the expression of Nestin in NSPCs after the treatment. (F, G) Flow cytometry was used to evaluate the effects of 0.8 μmol/L ART on cell cycle progression of NSPCs at 72h after treatment. Data are shown as the mean ± SEM, *p< 0.05, compared to untreated group.