Research Paper Volume 12, Issue 9 pp 8029—8048

Artesunate promotes the proliferation of neural stem/progenitor cells and alleviates Ischemia-reperfusion Injury through PI3K/Akt/FOXO-3a/p27kip1 signaling pathway

Figure 3. The proliferation of NSPCs subjected to oxygen-glucose deprivation/reperfusion was improved by ART. (A, B) After oxygen-glucose deprivation/reperfusion, CCK8 was used to examine the effects of various ART dosages (0, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8 and 25.6 μmol/L) on the proliferation of NSPCs at 24h and 72h. (C, D) Western blotting was used to detect the expression of Nestin in NSPCs after oxygen-glucose deprivation/reperfusion treated with ART (0, 0.4, 0.8 and 1.6 μmol/L). (E) RT-qPCR was used to determine the expression level of Nestin in NSPCs treated with ART 72h after oxygen-glucose deprivation/reperfusion. Data are shown as the mean ± SEM, *p<0.05; compared to untreated group.