Figure 4. PI3K/Akt/FOXO-3a/p27Kip1 signaling is involved in ART-induced proliferation of NSPCs after OGD/R injury. (A) 72h after OGD/R, western blotting was performed in control (OGD), ART-treated (OGD+ART) and ART and wortmannin-treated (OGD+ART+Wort) group. (B–E) The expression levels of p-AKT, p-FOXO-3a, p27kip1, and Nestin were examined. (F) RT-qPCR was used to evaluate the expression of Nestin in NSPCs of different groups. (G, H) CCK8 was used to examine the proliferation of NSPCs at 24 h and 72h after OGD/R injury. (I, J) Flow cytometric analysis was carried out to determine the cell cycle progression of NSPCs 72h post-OGD/R injury. Data are shown as the mean ± SEM (*p<0.05, **p<0.01, *** p<0.001 compared to OGD group; #p<0.05, ##p<0.01, ###p<0.001 compared to OGD+ART group).