Research Paper Volume 12, Issue 9 pp 8029—8048

Artesunate promotes the proliferation of neural stem/progenitor cells and alleviates Ischemia-reperfusion Injury through PI3K/Akt/FOXO-3a/p27kip1 signaling pathway

Figure 6. PI3K/Akt/FOXO-3a/p27Kip1 signaling is involved in ART-mediated cerebral infarct volume and neurologic impairment of MCAO animals. (A, B) The cerebral infarct volume was revealed by TTC staining at 72h after MCAO, and the cerebral infarct volume was quantified. (C) Neurological evaluation (18-point scale assessment of the impairment severity) was used to measure somatosensory movement function. (D) The ladder rung walking task was performed to evaluate fine motor function, and the slip ratio of contralateral limbs was counted within 50 steps. (E–I) Representative western blotting for PI3K/Akt/FOXO-3a signaling related molecules (p-AKT, p-FOXO-3a, p27kip1) and Nestin. Data are shown as the mean ± SEM. (*p<0.05, **p<0.01, *** p<0.001 vs. MCAO+Veh group; #p <0.05, ##p<0.01, ###p<0.001 vs. MCAO+ART group).