Research Paper Volume 12, Issue 9 pp 8067—8083

BMAL1 knockdown triggers different colon carcinoma cell fates by altering the delicate equilibrium between AKT/mTOR and P53/P21 pathways

Figure 5. P53 expression status in CRC BMAL1-KD cell lines. (A) Quantitative RT-PCR revealed that no significant change of P53 mRNA levels in the three CRC cell lines. 36B4 was used as a quantitative reference for all quantitative RT-PCR analyses (n=8). (B) Western-blot analysis revealed no significant change of P53 protein levels in the three CRC cell lines (n=7). (C and D) Cytoplasmic (C) and nuclear (D) extracts from BMAL1-KD and control cell lines were analyzed by western-blot. Only SW480 BMAL1-KD cells exhibited a significant decrease of cytoplasmic P53 (n=5; *p<0.05) associated with a significant increased nuclear P53 (n=5; *p<0.05). Left, a representative immunoblot of 5 independent experiments was shown. Right, Bar charts represented P53 expression level normalized to HSC70 or LAMIN B1. All data are shown as means ± SEM.