Figure 5. VA-CN promoted neuroprotection after SCI. (A) Co-labeled CD11b (green) immunoreactive, Ki67 marker (red) and Dapi (blue) in the spinal cord of rats. White arrows represent microglia, yellow arrows represent proliferated cells and red arrows represent the proliferation of microglia cells (Scale bar: 20 μm), n=6 per group. (B) Florescence images of NF160 in injured spinal cord at 28 day after SCI (Scale bar: 50 μm). (C, H) Representative images for IL-1β immunohistry (200× magnification) at 7 days after injury and the IL-1β positive cells were quantified. n=6 per group, * p<0.05, ** p<0.01, *** p<0.001. (D–F) Quantification of IL-1β, IL-6 and TNF-α production was evaluated at 7 days after injury, n=6 per group, * p<0.05, ** p<0.01, *** p<0.001. (G) Intensify quantification of NF160 florescence in injured spinal cord at 28 day after SCI, n=6 per group, * p<0.05, ** p<0.01.