Research Paper Volume 12, Issue 9 pp 8107—8119

Nur77 attenuates inflammatory responses and oxidative stress by inhibiting phosphorylated IκB-α in Parkinson’s disease cell model

Figure 3. Regulation of the Nur77 on anti-oxidant stress. (A) CSN-B treatment significantly reversed the reduction of Nrf2 caused by MPP+, siNur77 transfection reduced the expression of Nrf2 under MPP+ stress. (BD) CSN-B could significantly increase the expression of HO-1 and NQO-1, while Silencing of Nur77 with siRNA can significantly decrease HO-1 and NQO-1expression.(***P < 0.001, *P < 0.05 compared with control group; $P < 0.05.compared with MMP+ group; n=3, mean +/- SEM). (E) Immunohistochemistry of Nrf2 in PC12 cells after CSN-B treatment. (F, G) Protein expression and Quantitative analysis of Nrf2 in PC12 cell nucleus.