Research Paper Volume 12, Issue 9 pp 8137—8150

Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8

Figure 3. High-glucose regulated RPE cell functions by downregulating miR-25-3p. Western Blot was conducted to determine the expressions of (A, B) proliferation associated proteins (Cyclin D1, CDK2 and Cyclin E2), (C, D) apoptosis associated proteins (Bax, cleaved caspase-3 and Bcl-2) and (E, F) pyroptosis associated proteins (Caspase-1, Gasdermin D, NLRP3, IL-1β and IL-18) in RPE cells. (“Con” means “Control”, “Hg” means “High-glucose”, “Mic” means “miR-25-3p mimic” and “Inhi” means “miR-25-3p inhibitor”). Each experiment had at least 3 repetitions, the data were collected and represented as Mean ± SD. “*” means p < 0.05 and “**” means p < 0.01.