Research Paper Volume 12, Issue 9 pp 8137—8150

Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8

Figure 6. High-glucose inhibited RPE cell viability by regulating PTEN/Akt signal pathway. (A, B) Colony formation assay was performed to detect RPE cell proliferation. (C, D) FCM was used to determine RPE cell apoptosis ratio. (E, F) Western Blot was performed to determine the expression levels of pyroptosis associated proteins (Caspase-1, Gasdermin D, NLRP3, IL-1β and IL-18) in RPE cells. (“Hg” means “High-glucose”, “OE-M” means “overexpressed METTL3” and “OE-P” means “Overexpressed PTEN”). Each experiment had at least 3 repetitions, the data were collected and represented as Mean ± SD. “*” means p < 0.05 and “**” means p < 0.01.