Figure 3. Platelet spreading and clot retraction. Washed human platelets were placed on glass coverslips coated with fibrinogen (A) or collagen (B) at 37°C for 90 min followed by staining with Alexa Fluor-546-labelled phalloidin (mean ± SD, n = 3). Clot retraction was also performed in Salidroside-treated platelets (mean, n = 3) (C). Meanwhile, under clot retraction condition, the phosphorylation level of c-Src, Syk and PLCγ2 was measured by western blot and represented as a ratio relative to the total level (mean ± SD, n = 3) (D). For panel A, B and D, data were analyzed by one-way ANOVA. Compared with 0, *P < 0.05; **P < 0.01; ***P < 0.001. For panel C, data were analyzed by two-way ANOVA. Compared with 10 or 20, ***P < 0.001.