Research Paper Volume 12, Issue 9 pp 8151—8166

Salidroside inhibits platelet function and thrombus formation through AKT/GSK3β signaling pathway

Figure 6. Deep vein thrombus formation and coagulation analysis. After intraperitoneal injection of salidroside (20 mg/kg) or vehicle, mice underwent ligation of inferior vena cava (IVC) to initiate venous thrombus formation. After 24 h, the IVC samples (A, representative IVC from 5 mice) were collected for measuring the thrombus weight (B) and length (C) (n = 5). Meanwhile, the histological assessment of the ligated IVC samples was also performed (magnification x 40, scale bar =1000 μm) (D). In addition, peripheral blood was collected from salidroside or vehicle treated mice for analysis of coagulation factor FVIII (E), FIX (F) and prothrombin time (G) (n = 7). Data were presented as mean ± SE and analyzed by unpaired student t-test.