Figure 5. R406 inhibits phosphorylation of FAK in senescent HDFs more than non-senescent cells. (A, B) Senescent (S) and non-senescent (NS) HDFs were treated with R406 (10 μM, A), or ABT263 (1 μM, B) for 1 h and then western blot assays with anti-p-FAK and FAK antibodies. (C, D) Senescent HDFs were respectively treated with DMSO, R406 (10 μM), and PF562271 (5 μM) for one day and then western blot assay (anti-p-FAK and caspase-3, C) and apoptosis assay with Annexin V/PI staining (D) were conducted to determine senolytic effect. (E, F) Western blot (E) and apoptosis assay (F) with non-senescent HDFs as described in C and D. *p<0.05, **p<0.01, ***P<0.001.