Research Paper Volume 12, Issue 9 pp 8221—8240

Identification of SYK inhibitor, R406 as a novel senolytic agent

Figure 6. R406 inhibits phosphorylation of p38 MAPK in senescent HDFs more than non-senescent HDFs. (A, B) Senescent (S) and non-senescent (NS) HDFs were treated with DMSO, R406 (10 μM), and ABT263 (1 μM) for one day and western blot assays using anti-MAPKs antibodies were conducted. (C, D) Senescent HDFs were respectively treated with DMSO, R406 (10 μM), and SB202190 (p38 inhibitor, 50 μM) for one day. Then, western blot assays were conducted to determine triggering apoptosis by caspase cleavage. *p<0.05, **p<0.01, ***P<0.001.