Research Paper Volume 12, Issue 9 pp 8221—8240

Identification of SYK inhibitor, R406 as a novel senolytic agent

Figure 7. R406 induces senolytic effects via blocking the phosphorylation of p38 MAPK and FAK simultaneously. (A) Senescent (S) and non-senescent (NS) HDFs were respectively treated with DMSO, R406 (10 μM), PF562271 (5 μM), and SB202190 (50 μM) for one day and then western blot assay with anti-caspase-9 antibody was conducted to determine apoptosis. (B) Proposed mechanism of senolytic effect by R406. R406-induced cell death is mediated by inhibiting FAK and p38 activity as well as increasing ROS. *p<0.05, **p<0.01, ***P<0.001.