Research Paper Volume 12, Issue 9 pp 8241—8260

Long-noncoding RNA MALAT1 sponges microRNA-92a-3p to inhibit doxorubicin-induced cardiac senescence by targeting ATG4a

Figure 2. LncRNA-MALAT1 transferred by exosomes to cardiomyocytes. (A) Heat map of the lncRNAs differentially expressed between exosomes derived from MSCs pretreated with hypoxia (exosomeHypoxia) and exosomes derived from MSCs without any treatment (exosome). (B) Relative lncRNA-MALAT1 expression was validated by qRT-PCR in exosomeHypoxia and exosome; *P < 0.05 versus exosome; (C) LncRNA-MALAT1 mRNA was examined by qRT-PCR in cardiomyocytes incubated with exosomeHypoxia or exosome. The cardiomyocytes without any treatment were used as control; *P < 0.05 versus control; P < 0.05 versus exosomeHypoxia. (D) The siRNA-mediated transfection efficiency in MSCs was demonstrated by qRT-PCR. *P < 0.05 versus siRNA-lncRNA-MALAT1. (E) LncRNA-MALAT1 mRNA in exosomes was examined by qRT-PCR. Each column represents the mean ± SD of three independent experiments. *P < 0.05 versus control; P < 0.05 versus hypoxia + siRNA-lncRNA-MALAT1. (F) LncRNA-MALAT1 mRNA in cardiomyocytes was examined by qRT-PCR. *P < 0.05 versus control; P < 0.05 versus exosomeHypoxia; °P < 0.05 versus exosomeHypoxia+siRNA-LncRNA-NT