Research Paper Volume 12, Issue 9 pp 8241—8260

Long-noncoding RNA MALAT1 sponges microRNA-92a-3p to inhibit doxorubicin-induced cardiac senescence by targeting ATG4a

Figure 3. LncRNA-MALAT1 transferred by exosomes caused rejuvenation against Dox. (A) LncRNA-MALAT1 mRNA in cardiomyocytes was examined by qRT-PCR. *P < 0.05 versus Control; P < 0.05 versus Dox. (B) Cell cycle distribution was analyzed. (C and D) p53 and p21 mRNA levels were analyzed by RT-qPCR. (E) Representative images of SA-β-gal staining. (F) Percentage of β-gal-positive cells. Each column represents the mean ± SD of three independent experiments. *P < 0.05 versus control; P < 0.05 versus Dox; P < 0.05 versus Dox + exosomeHypoxia+siRNA-lncRNA-MALAT1.