Research Paper Volume 12, Issue 9 pp 8241—8260

Long-noncoding RNA MALAT1 sponges microRNA-92a-3p to inhibit doxorubicin-induced cardiac senescence by targeting ATG4a

Figure 4. LncRNA-MALAT1 directly inhibited miR-92a-3p. (A) Heat map of microRNAs (miRs) differentially regulated by exosomeHypoxia in Dox-treated cardiomyocytes. (B) qRT-PCR validation of the differentially regulated miRs in cardiomyocytes. *P < 0.05 versus Dox. (C) Binding sites of lncRNAs and miRs. (D) Dual-luciferase reporter. *P < 0.05 versus miR-92a-3p mimic in the WT group. (E) miR-92a-3p mRNA levels were analyzed by RT-qPCR. *P < 0.05 versus control; P < 0.05 versus Dox; P<0.05 versus Dox + exosomeHypoxia+siRNA-lncRNA-MALAT1.