Research Paper Volume 12, Issue 9 pp 8261—8288

Identification of candidate lncRNAs and circRNAs regulating WNT3/β-catenin signaling in essential hypertension

Figure 7. LOC646616 increases WNT3 expression by sponging miR-637. (A) qRT-PCR analysis of LOC646616 expression in HASMCs transfected with control siRNA (si-NC) or with 3 siRNA variants targeting LOC646616. (B, C) Results of Transwell invasion and viability (CCK-8) assays conducted in HASMCs transfected with si-LOC646616-2. (D) Left panel: relative miR-637 expression in HASMCs transfected with si-NC or si-LOC646616-2. Right panel: relative WNT3 mRNA expression in HASMCs transfected with NC or anti-miR-637. (E, F) WNT3 mRNA (left panel) and protein (middle and right panel) levels in HASMCs following knockdown of LOC646616 (si-LOC-2) with/without concurrent inhibition of miR-637. *P<0.05, **P<0.01, ***P<0.001.