Figure 2. Targeting relationship between CCAT1 and miR-181a in HT-29 cells. (A) The CCAT1 expression levels in four CRC cell lines (CW-2, SW-620, HCT 116 and HT-29) and the human normal colorectal cell NCM-460 were detected by qRT-PCR. The expression of CCAT1 was normalized to that in NCM-460. (B) HT-29 was transfected with two different siRNAs against CCAT1. (C) miR-124, miR-490-3p, miR-194, miR-24 and miR-181a-5p expression in HT-29 cells. (D) MiR-181a-5p was downregulated in 50 paired CRC tissues and adjacent tissues. (E) Putative miR-181a-5p binding sequence of CCAT1 is shown. (F) The relative luciferase activity was detected in HT-29 cells co-transfected with CCAT1-WT or CCAT1-MUT and miR-181a-5p or miR-NC. All assays were performed three times. **P<0.01, ***P <0.001, compared with NC group.