Research Paper Volume 12, Issue 9 pp 8301—8320

Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression

Figure 7. LncRNA CCAT1 influenced colorectal cancer cell apoptosis through targeting miR-181a-5p. (A, B) The apoptosis rate of HT-29 cells transfected with si-CCAT1-1, si-CCAT1-2 or miR-181a-5p mimics dramatically increased, whereas that of cells transfected with miR-181a-5p inhibitor remarkably decrease detected by flow cytometry. (C, D) Western blot analysis of p53 protein level and apoptosis-related protein Bax and Bcl-2 expression levels in HT-29 cells. The bar chart illustrated the level of p53 and Bax were significantly higher than NC group after CCAT1 knockdown and miR-181a-5p overexpression. All assays were performed three times. *P<0.05, **P<0.01, ***P<0.001, compared with NC group.