Research Paper Volume 12, Issue 21 pp 21076—21090

Downregulation of interleukin-6 and C-reactive protein underlies a novel inhibitory role of microRNA-136-5p in acute lower extremity deep vein thrombosis

Figure 3. Acute LEDVT is mitigated by the upregulation of miR-136-5p. The rats used for following assays included rats injected with normal saline alone, sham-operated rats, acute LEDVT model rats, and rats treated with miR-136-5p agomir or miR-136-5p antagomir. (A) The expression levels of miR-136-5p, IL-6 and CRP in the vein tissues of rats determined by RT-qPCR. (B) The protein expression levels of IL-6 and CRP in peripheral blood serum of rats determined by ELISA. (C) Images of acute LEDVT in rats. (D) The length, weight, and the ratio of weight to length of the venous thrombus in rats. (E) The pathological changes in vein tissues of rats determined by HE staining (100 ×). (F) The apoptosis of endothelial cells in the femoral vein in rats measured by TUNEL assay (200 ×). ** p < 0.01 compared with sham-operated rats; # p < 0.05 compared with the acute LEDVT model rats. Measurement data were expressed as mean ± standard deviation. Data from multiple groups were compared using one-way ANOVA. N = 12 for rats in each group. LEDVT, lower extremity deep vein thrombosis; miR-136-5p, microRNA-136-5p; IL-6, interleukin-6; CRP, C-reactive protein; RT-qPCR, reverse transcription quantitative polymerase chain reaction; ELISA, enzyme linked immunosorbent assay; HE, hematoxylin-eosin; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling; ANOVA, analysis of variance.