Research Paper Volume 12, Issue 9 pp 8321—8338

Mitochondria-related miR-574 reduces sperm ATP by targeting ND5 in aging males

Figure 3. Overexpression of miR-574 impaired mitochondrial function and reduced cellular ATP production. (A) MiR-574 overexpression efficiency detection in GC2 cells transfected with miR-574 mimic. (B) Overexpression of miR-574 decreased intracellular ATP levels. (C) The mitochondrial membrane potential of the miR-574 overexpression group was significantly inhibited compared with control groups, as assayed by flow cytometry. (D) MiR-574 increased the intracellular ROS levels in GC2 cells. Scale bar=100 μm. (E) Immunofluorescence was used to detect intracellular 8-OHdG levels. MiR-574 significantly increased intracellular 8-OHdG (green) levels compared with the control group. The nuclei were stained blue with 4,6-diamidino-2-phenylindole (DAPI). Scale bar=100 μm.