Research Paper Volume 12, Issue 10 pp 8968—8986

Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair

Figure 6. Knockdown of miR-15a-3p partially reversed the negative effects of Dia-Exos on wound healing in vivo. (A) General view of the wounds among in the four groups on days 0, 3, 5, 7, 10 and 14 post-wounding; n = 6 per group. (B) The mice of wound closure in the four groups were quantified using digital images evaluated with ImageJ software; n = 6 per group. (C, D) The blood flow at the wounds in the four groups was evaluated using small animal doppler detection; n = 6 per group. (E) The expression of miR-15a-3p in the wound tissues of the four groups. (F, G) CD31 immunohistochemistry results of the four groups. The number of CD31-positive cells was quantified in five random fields of view. Vessels with diameters of 2-10 μm were counted as individual vessels. n = 6 per group; scale bar: 100 μm. Data are the means ± SDs of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.