Research Paper Volume 12, Issue 9 pp 8352—8371

Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1

Figure 1. Exosomes secreted from CRC cells exhibit high expression of miR-183-5p. (A) heatmap of differentially expressed miRNA screened from GSE108153, with the horizontal coordinate representative of the sample number; the longitudinal coordinate reflective of the differentially expressed miRNA; the color gradation histogram on the right indicative the level of gene expression where each box in the diagram indicates the expression level of a gene in a sample. The warmer color reflected the higher expression, and the colder color reflected the lower expression; (B) expression of miR-183-5p in CRC cell lines detected via RT-qPCR; (C) identification of structure of exosomes by transmission electron microscopy; (D) analysis of size distribution of exosomes was conducted by NanoSight NS300 Nanoparticle Tracking Analyzer; (E) protein bands and expression of TSG101, CD63 and GRP94 as detected by Western blot analysis; (F) expression of miR-183-5p in exosomes secreted from CRC cells detected via RT-qPCR; * p < 0.05 compared with the FHC cell. Measurement data were expressed as mean ± standard deviation; comparisons among multiple groups were assessed by one-way analysis of variance. Cell experiment was repeated three times.