Research Paper Volume 12, Issue 9 pp 8352—8371

Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1

Figure 6. Inhibition of miR-183-5p in HT29 cells reduces the expression of FOXO1 and angiogenesis-related factors in nude mice. (A) expression of miR-183-5p in serum detected by RT-qPCR; (B, C) immunohistochemical detection of FOXO1 expression in tumor tissues of nude mice (Scale bar = 25 μm); (DE) expression of angiogenesis-related proteins (VEGFA, VEGFAR2, ANG2, PIGF, MMP-2 and MMP-9) and FOXO1 in tumor tissues detected by Western blot analysis; *p < 0.05 compared with the HT29-NC group; Measurement data were presented as mean ± standard deviation; comparisons between two groups were conducted by means of independent t-test, n = 10.