Research Paper Volume 12, Issue 9 pp 8372—8396

KIF23 activated Wnt/β-catenin signaling pathway through direct interaction with Amer1 in gastric cancer

Figure 2. KIF23 knockdown suppressed cell cycle progression of GC cells. (A) A public dataset (GSE65801) was divided into two groups—a high KIF23 expression group and a low KIF23 expression group—and the normalized enrichment score (NES) of three gene set categories was calculated by gene set enrichment analysis (GSEA). (B) Cell cycle and (C) BrdU incorporation assay analysis of GC cells transfected with siRNAs against KIF23. The percentages of the cell subpopulations at different stages of the cell cycle were statistically analyzed. (D) Immunofluorescence images showed the morphology of GC cells after being transfected with siRNAs.