Research Paper Volume 12, Issue 9 pp 8372—8396

KIF23 activated Wnt/β-catenin signaling pathway through direct interaction with Amer1 in gastric cancer

Figure 5. KIF23 combined with the membrane localization and APC-binding domains within Amer1. (A) Amer1-FL (full length) and its three truncation mutants were constructed, namely, ΔM (lacking the N-terminal membrane localization domains, MLD), ΔA (lacking the APC-binding domains, ABD) and ΔMA (lacking the membrane localization domains and APC-binding domains). Coimmunoprecipitation (B) and GST-pull down (C) of FLAG-tagged Amer1 with eGFP-tagged KIF23 after transient transfection of 293T cells as indicated. Immunofluorescence staining (D) and western blot analysis (E) for the distribution of Amer1 and KIF23 in MGC-803 cells after transfection with eGFP-tagged KIF23 or control plasmid and FLAG-tagged Amer1-FL or its three truncation mutants.