Research Paper Volume 12, Issue 9 pp 8473—8483

Shoutai pills improve the quality of oocytes exposed to the chemotherapeutic drug Hydroxyurea

Figure 3. Effects of STP on the spindle assembly and chromosome alignment in HU-exposed oocytes. (A) Representative images of spindle morphology and chromosome alignment in control, HU-exposed, and STP-supplemented oocytes. M I Oocytes were immunostained with α-tubulin-FITC antibody to visualize the spindles and counterstained with Hoechst to visualize the chromosomes. Scale bar, 2.5 μm. (B) The rates of aberrant spindles were recorded in control, HU-exposed, and STP-supplemented oocytes. (C) The rates of misaligned chromosomes were recorded in control, HU-exposed, and STP-supplemented oocytes. Data of (B, C) were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01.