Figure 4. Effects of STP on the kinetochore-microtubule attachment in HU-exposed oocytes. (A) Representative images of kinetochore-microtubule attachment in control, HU-exposed, and STP-supplemented oocytes. Oocytes were immunostained with α-tubulin-FITC antibody to visualize the spindles, with CREST to display the kinetochores, and counterstained with Hoechst to visualize the chromosomes. Scale bar, 5 μm. (B) The rates of defective kinetochore-microtubule attachments were recorded in control, HU-exposed, and STP-supplemented oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, ***P < 0.001.