Research Paper Volume 12, Issue 9 pp 8473—8483

Shoutai pills improve the quality of oocytes exposed to the chemotherapeutic drug Hydroxyurea

Figure 7. Effects of STP on the ROS level, DNA damage and early apoptosis in HU-exposed oocytes. (A) Representative images of ROS signals in control, HU-exposed, and STP-supplemented oocytes. Scale bar, 20 and 40 μm. (B) The fluorescence intensity of ROS in control, HU-exposed and STP-supplemented oocytes was measured by confocal microscopy using identical settings and parameters. (C) Representative images of γH2AX foci in control, HU-exposed, and STP-supplemented oocytes. Scale bar, 5 μm. (D) The fluorescence intensity of γH2AX signals was measured in control, HU-exposed, and STP-supplemented oocytes. (E) Representative images of apoptotic oocytes in control, HU-exposed, and STP-supplemented oocytes. Scale bar, 20 μm. (F) The rates of apoptotic oocytes were recorded in control, HU-exposed, and STP-supplemented oocytes. Data of (B), (D), and (F) were presented as mean percentage (mean ± SD) of at least three independent experiments. *P < 0.05, ***P < 0.001.