Research Paper Volume 12, Issue 9 pp 8549—8564

Exosomal miRNA-34 from cancer-associated fibroblasts inhibits growth and invasion of gastric cancer cells in vitro and in vivo

Figure 6. Exosomes mediate intercellular communication between GCFs and GC cells AZ521. (A) The mRNA level of Cre in exosomes derived from GCFs transfected with Cre vectors or negative control. (B) The morphology of exosomes derived from GCFs and the diameter distribution, as determined by transmission electron microscopy and nanoparticle tracking analysis. (C) The protein expressions of exosomal markers, CD63 and TSG101, in exosomes derived from GCFs. (D) GCFs-derived exosomes were internalized by GC AZ521 cells. Exosomes were isolated from GCFs labeled with Cre and then cocultured with AZ521—loxp-dsRed-loxp-Stop-eGFP for seven days. The color switching from red to green indicated that exosome-carrying Cre was transferred to the cytoplasm of AZ521 cells. Scale bars: 50 μm. Values are means ± SD; ***, P < 0.001.