Research Paper Volume 12, Issue 10 pp 9000—9013

The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress

Figure 1. The successful infection of the C3H10T1/2 cells with Ad-SIRT1, Ad-BMP2, and Ad-GFP. (A) C3H10T1/2 cells were infected with Ad-SIRT1, Ad-BMP2, or Ad-GFP in plate cultures. Bright light and fluorescence microscopy (100X) examination showed the efficiency of recombinant adenoviruses after 24 h of virus infection. (B, C) Total RNA was isolated 24 h after infection, and the mRNA expression of SIRT1 and BMP2 was measured using real-time PCR. (D, E) The SIRT1 and BMP2 protein levels were measured after 48 h of infection using the western blotting method. The data are denoted as the mean ± SD.*: p < 0.05 vs. Ad-GFP.