Research Paper Volume 12, Issue 10 pp 9000—9013

The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress

Figure 5. SIRT1 alleviated the apoptosis of BMP2-induced chondrogenic differentiation stem cells under oxidative stress. (A) Cell Counting Kit-8 assay to detect cell proliferation under different concentrations (0, 50, 100, 200, or 400 μM) of H2O2. (B) The cell apoptotic rates were measured using a flow cytometry assay under 200 μM of H2O2. (C, D) H2O2 increased the expression of Cleaved-Caspase3, Bax, and Cleaved-PARP-1 and reduce the expression of Bcl-2 compare to the control group. The Ad-BMP2+Ad-SIRT1+H2O2 group exhibited opposite results. The data are denoted as the mean ± SD.*: p < 0.05 Ad-BMP2+H2O2 vs. Ad-BMP2; #: p < 0.05, Ad-BMP2 + H2O2 vs. Ad-BMP2+Ad-SIRT1+H2O2.