Figure 7. Detection of pulmonary vascular miR-125a-5p and HK-II expression. (A) RT-PCR assay showed that the expression of miR-125a-5p in PASMCs was significantly lower in PH group. The level of miR-125a-5p expression in PH+ agomir group was higher than that in PH group. (B) WB results showed that the expression of HK-II was significantly increased in the PH group, thus demonstrating that miR-125a-5p negatively regulates the expression of HK-II in PASMCs. (*P<0.05, compared with control group, #P<0.05, compared with PH group).