Research Paper Volume 12, Issue 13 pp 12726—12739

EZH2-mediated inhibition of microRNA-22 promotes differentiation of hair follicle stem cells by elevating STK40 expression

Figure 2. STK40 expedites the proliferation and differentiation of HFSCs via MEF2-ALP axis. (A) Expression of STK40, MEF2, ALP, differentiation-related proteins (β-catenin, TCF-4), and TA cell differentiation markers (CK15, CK19) normalized to GAPDH determined by Western blot analysis. (B) Colony forming capacity of HFSCs determined by colony formation assay. (C) HFSC proliferation and viability evaluated by MTS assay. (D) HFSC cell cycle changes assessed by flow cytometry. * p < 0.05 vs. WT mice; # p < 0.05 vs. si-NC (WT) group; $ p < 0.05 vs. oe-NC (STK40-/-) group; Measurement data were expressed as mean ± standard deviation. One-way ANOVA was utilized to compare data among multiple groups, followed by Tukey’s post hoc test. Cell experiments were conducted in triplicates.