Figure 6. EZH2 inhibits miR-22 expression to elevate STK40, which stimulates MEF2-ALP activity. (A) The expression of miR-22, and mRNA expression of STK40, MEF2, ALP in HFSCs determined using RT-qPCR. (B) Protein expression of STK40, MEF2 and ALP in HFSCs normalized to GAPDH measured by Western blot analysis. (C) Enrichment of EZH2 or H3K27me3 in miR-22 promoter region evaluated using ChIP assay. * p < 0.05 vs. si-NC group; # p < 0.05 vs. oe-NC group; Measurement data were expressed as mean ± standard deviation. One-way ANOVA was utilized to compare data among multiple groups, followed by Tukey’s post hoc test. Cell experiments were conducted in triplicates.