Figure 8. EZH2 knockout inhibits HF keratinocyte differentiation and hair growth, but facilitated apoptosis in vivo. (A) Hair loss exhibited by EZH2-/- mice of 30 days old. (B) Delayed hair growth ability in EZH2-/- mice. (C) HF in EZH2-/- mice and WT mice determined by ALP staining. (D) Expression of proliferation, differentiation, and apoptosis markers EZH2-/- mice and WT mice detected by immunofluorescence assay (400 ×). * p < 0.05 vs. WT mice; Measurement data were expressed as mean ± standard deviation. Unpaired t test was adopted to analyze the differences between two experimental groups if the data conformed to normal distribution and homogeneity of variance. n = 15.