Research Paper Volume 12, Issue 10 pp 9066—9084

Overexpression of miR-142-5p inhibits the progression of nonalcoholic steatohepatitis by targeting TSLP and inhibiting JAK-STAT signaling pathway

Figure 2. JAK-STAT pathway was actived in NASH patients and verification of the target gene. (A) Dotplot suggest the distributions of some biological pathway gene sets. The size of the circle represents the count value. The gradient changes of color represented adjust P values. (B) KEGG analysis of JAK-STAT pathway in NASH. (C) Expression of TSLP in NASH disease model. *P < 0.05, compared with control group or WT group. (D) Venn diagram showing the overlap between dysregulated miRNAs and miRNAs that target at TSLP. (E) RIP assays of the enrichment of Ago2 on TSLP and miR-142-5p relative to IgG in liver. **P < 0.01, compared with IgG group. (F) The miR-142-5p binding sites on TSLP were predicted by bioinformatics. TSLP wild-type form (TSLP-wt) and mutated form (TSLP-mut) were displayed on the left panel. Dual-luciferase reporter assay was conducted to identify the target relationship between miR-142-5p and TSLP. All data were means ± SD.