Figure 3. TNF decreases PAK4 gene and protein levels in HUVECs. (A) Western blot analysis of PAK4 and IκBα in HUVECs stimulated with 100 ng/ml TNF without and with 300 ng/ml soluble TNFR1. (B) Quantitative real-time PCR of PAK4 mRNA in HUVECs treated with 100 ng/ml TNF. All data are expressed as the mean + SD; *** P<0.001, compared with untreated cells.