Research Paper Volume 12, Issue 13 pp 12740—12749

PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells

Figure 3. TNF decreases PAK4 gene and protein levels in HUVECs. (A) Western blot analysis of PAK4 and IκBα in HUVECs stimulated with 100 ng/ml TNF without and with 300 ng/ml soluble TNFR1. (B) Quantitative real-time PCR of PAK4 mRNA in HUVECs treated with 100 ng/ml TNF. All data are expressed as the mean + SD; *** P<0.001, compared with untreated cells.