Figure 6. PAK4 inhibits caspase activation and apoptosis in HUVECs. (A) Active caspase-3-FITC staining analyzed by FC in HUVECs transfected 48 h with GFP-PAK4 or PAK4 siRNA, and stimulated with TNF (100 ng/ml). (B) Quantitative evaluation of apoptotic cells analyzed by FC in panel A. The data represent the mean ± s.d. of three independent experiments. P-values were calculated using Student’s t-test. * P<0.05, ** P<0.01. (C) Western analysis of total caspase-8, active caspase-8, and control tubulin in TNF-stimulated HUVECs transfected with GFP-PAK4 or PAK4 siRNA.