Figure 5. Prostate cell lines exhibiting downregulated MXRA5 protein. (A) Representative immunofluorescence staining of MXRA5 (red) in the WPMY-1 cells after si-MXRA5-3 treatment, compared with si-control treatment. Nuclei were stained by DAPI (blue). Three repeats of experiments for were conducted and representative graphs were selected into figure. The scale bar is 100 μm. (B) qRT-PCR validated the efficiency of distinct si-RNAs to knockdown MXRA5 at the transcriptional level in the prostate cells WPMY-1. All values shown are mean ± SD of triplicate measurements and repeated three times with similar results, * means P<0.05 and ** means P<0.01. (C) Western blot analysis showed a decreased protein expression of MXRA5 by the si-MXRA5 treatments, comparing to si-control treatment. (D) Relative densitometric quantification of MXRA5 in WPMY-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. * means P<0.05 and ** means P<0.01 vs. si-control.