Research Paper Volume 12, Issue 9 pp 8605—8621

Identification and functional activity of matrix-remodeling associated 5 (MXRA5) in benign hyperplastic prostate

Figure 9. Upregulation of MXRA5 promotes prostate cell proliferation. (A, B) Flow cytometry analysis for BPH-1 cells treated with MXRA5 plasmid (Panel A: Right) for 48h compared with vector-control treated cells (Panel A: Left). Percentages (%) of cell populations at different stages of cell cycles were listed within the panels. All histograms revealed the percentage (%) of cell populations from three independent experiments, * means P < 0.05. (C) Cell proliferation of BPH-1 cells treated by vector-control (Left) and MXRA5 plasmid (Right) was detected by Ki-67 staining (green). Nuclei were stained by DAPI (blue). Three repeats of experiments for were conducted and representative graphs were selected into figure. The scale bar is 100 μm. (D) MTT assay was used to detect the viability of the BPH-1 cells treated by vector-control (black line) and MXRA5 plasmid (red line). (E) Flow cytometry analysis of alterations of BPH-1 cells apoptosis via transfection using vector-control (Left) and MXRA5 plasmid (Right). Calculation area of the apoptosis rate was percentage of Annexin V+/PI+ cells. (F) Statistical analysis suggested no significant (ns) induction of apoptosis by the upregulation of MXRA5 in BPH-1 cells. All values shown are mean ± SD of triplicate measurements and repeated three times with similar results.