Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 1. Upregulation of lncRNA ZFAS1 in PF is positively correlated with SLC38A1 expression. (A, B) RT-qPCR was performed to detect the expression of lncRNA ZFAS1 and SLC38A1 in lung tissues; (C) Spearman analysis was used to analyze the association between lncRNA ZFAS1 and SLC38A1 expression in the lung tissues of BLM-induced pulmonary fibrosis cases; (D, E) The expression of lncRNA ZFAS1 and SLC38A1 in HFL1 cells treated with TGF-β1 or control were determined by RT-qPCR; (F) RT-qPCR was used to measure the expression of lncRNA ZFAS1 in either the nucleus or cytoplasm of HFL1 cells; (G) FISH was performed to evaluate the location of endogenous lncRNA ZFAS1 (green) in HFL1 cells, U6 and GAPDH were used as nuclear and cytoplasmic localization markers, respectively. DNA (blue) was stained with DAPI. ***P<0.001, compared with the control group; ###P<0.001, compared with the NC group.