Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 2. Knockdown of lncRNA ZFAS1 inhibits the FMT process in TGF-β1-induced HFL1 cells. (A) The expression of lncRNA ZFAS1 in HFL1 cells transfected with lncRNA ZFAS1 shRNA was determined by RT-qPCR; (BD) BrdU staining was applied to test cell viability; (CE) The migration ability of HFL1 cells was measured by wound healing assay; (F, G) Western blot was performed to detect the expression levels of E-cadherin, collagen I, FN1 and the FMT marker α-SMA; (H, I) Immunofluorescence staining was applied to evaluate the expression of α-SMA and E-cadherin in HFL1 cells. ***P<0.001, compared with the sh-NC group; ##P<0.01, ###P<0.001, compared with the control group; ΔΔP<0.01, ΔΔΔP<0.001, compared with the TGF-β1-treated group.