Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 3. Knockdown of lncRNA ZFAS1 alleviates TGF-β1-induced ferroptosis in HFL1 cells. (A) RT-qPCR was used to detect the mRNA expression of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in HFL1 cells; (B) The level of ROS was measured by ROS kit; (C) The MDA content was measured by a lipid peroxidation (MDA) assay kit; (D, E) Western blot was performed to detect the level of GPX4 protein. **P<0.01, ***P<0.001, compared with the control group; ##P<0.01, ###P<0.001, compared with the TGF-β1 group.